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transport media tube  (Copan Diagnostics)


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    Copan Diagnostics transport media tube
    Transport Media Tube, supplied by Copan Diagnostics, used in various techniques. Bioz Stars score: 97/100, based on 3141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transport media tube/product/Copan Diagnostics
    Average 97 stars, based on 3141 article reviews
    transport media tube - by Bioz Stars, 2026-06
    97/100 stars

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    Surrogate virus characteristics and specifics used for PSMTM inactivation studies.

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: Surrogate virus characteristics and specifics used for PSMTM inactivation studies.

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Virus, Modification

    PSMTM effectively inactivates diverse animal viruses while maintaining the detection of nucleic acid. (A) Virus recovery after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control. Virus recovery was assessed by TCID 50 titer using the cell line described in . (B) Nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation control. (C) Serial passage endpoint nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation. The serial passage was completed using the cell line in and the description outlined in Methods. Nucleic acid was detected according to the reference in and the description in the Methods section. PSMTM-treated viruses were compared to the corresponding no-PSMTM control, and inactivation was assessed using the manufacturer’s recommended conditions. Significance was determined using Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI); swine influenza virus (SIV); Newcastle disease virus (NDV); eastern equine encephalitis virus (EEEV); and bovine viral diarrhea virus (BVDV).

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: PSMTM effectively inactivates diverse animal viruses while maintaining the detection of nucleic acid. (A) Virus recovery after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control. Virus recovery was assessed by TCID 50 titer using the cell line described in . (B) Nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation control. (C) Serial passage endpoint nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation. The serial passage was completed using the cell line in and the description outlined in Methods. Nucleic acid was detected according to the reference in and the description in the Methods section. PSMTM-treated viruses were compared to the corresponding no-PSMTM control, and inactivation was assessed using the manufacturer’s recommended conditions. Significance was determined using Student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI); swine influenza virus (SIV); Newcastle disease virus (NDV); eastern equine encephalitis virus (EEEV); and bovine viral diarrhea virus (BVDV).

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Virus, Control

    Log10 TCID 50 <xref ref-type= a reduction and qRT-PCR cycle threshold (CT) change in PSMTM b treatment vs. control treatment viruses." width="100%" height="100%">

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: Log10 TCID 50 a reduction and qRT-PCR cycle threshold (CT) change in PSMTM b treatment vs. control treatment viruses.

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Control, Virus, Modification

    PSMTM to virus ratio determines the effectiveness of inactivation. Virus recovery and nucleic acid detection were determined after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control utilizing various treatment to virus ratio conditions: (A) 1:1 ratio; (B) 1:3 ratio; (C) 1:10 ratio; and (D) 1:100 ratio. Virus recovery was assessed by TCID 50 titer using the cell line described in . Nucleic acid was detected according to the reference in and the description in the Methods section. PSMTM-treated viruses were compared to the corresponding no-PSMTM control. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Eastern equine encephalitis virus (EEEV).

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: PSMTM to virus ratio determines the effectiveness of inactivation. Virus recovery and nucleic acid detection were determined after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control utilizing various treatment to virus ratio conditions: (A) 1:1 ratio; (B) 1:3 ratio; (C) 1:10 ratio; and (D) 1:100 ratio. Virus recovery was assessed by TCID 50 titer using the cell line described in . Nucleic acid was detected according to the reference in and the description in the Methods section. PSMTM-treated viruses were compared to the corresponding no-PSMTM control. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Eastern equine encephalitis virus (EEEV).

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Virus, Control

    Virus inactivation and nucleic acid detection are stable over time in PSMTM. Virus recovery and nucleic acid detection over 21 days for viruses deposited in a plastic screw-cap tube at ambient temperature. Inactivation was assessed using the manufacturer’s recommended conditions. (A) Virus recovery was assessed by TCID 50 for PBS no-inactivation control-treated LPAI H7N3 (left) and PrimeStore® molecular transport media (PSMTM)-treated LPAI H7N3 (right). (B) Virus nucleic acid cycle threshold (CT) detection for PBS no-inactivation control and PSMTM-treated LPAI H7N3. (C) Virus recovery was assessed by TCID 50 for PBS no-inactivation control-treated vaccinia virus (left) and PSMTM-treated vaccinia virus (right). (D) Virus nucleic acid cycle threshold (CT) detection for PBS no-inactivation control and PSMTM-treated vaccinia virus. (A,C) TCID 50 titer was determined in the appropriate cell line as identified in . Each timepoint was compared to timepoint 0 for each respective treatment. (B,D) Nucleic acid was detected according to the reference in and the description in Methods. PSMTM-treated viruses were compared to the corresponding no-PSMTM control. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI).

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: Virus inactivation and nucleic acid detection are stable over time in PSMTM. Virus recovery and nucleic acid detection over 21 days for viruses deposited in a plastic screw-cap tube at ambient temperature. Inactivation was assessed using the manufacturer’s recommended conditions. (A) Virus recovery was assessed by TCID 50 for PBS no-inactivation control-treated LPAI H7N3 (left) and PrimeStore® molecular transport media (PSMTM)-treated LPAI H7N3 (right). (B) Virus nucleic acid cycle threshold (CT) detection for PBS no-inactivation control and PSMTM-treated LPAI H7N3. (C) Virus recovery was assessed by TCID 50 for PBS no-inactivation control-treated vaccinia virus (left) and PSMTM-treated vaccinia virus (right). (D) Virus nucleic acid cycle threshold (CT) detection for PBS no-inactivation control and PSMTM-treated vaccinia virus. (A,C) TCID 50 titer was determined in the appropriate cell line as identified in . Each timepoint was compared to timepoint 0 for each respective treatment. (B,D) Nucleic acid was detected according to the reference in and the description in Methods. PSMTM-treated viruses were compared to the corresponding no-PSMTM control. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI).

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Virus, Control

    Presence of biological material does not affect the effectiveness of PSMTM on the inactivation or nucleic acid detection of diverse viruses. Viruses were combined with species-appropriate sera or cloacal swabs as described in the Methods section. (A) Virus recovery after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control. Virus recovery was assessed by TCID 50 titer using the cell line described in . (B) Nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation control. (C) Serial passage endpoint nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation. Serial passage was completed using the cell line in and the description outlined in the Methods section. Nucleic acid was detected according to the reference in and the description in Methods. PSMTM-treated viruses were compared to the corresponding no-PSMTM control, and inactivation was assessed using the manufacturer’s recommended conditions. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI); swine influenza virus (SIV); Newcastle disease virus (NDV); eastern equine encephalitis virus (EEEV); and bovine viral diarrhea virus (BVDV).

    Journal: Frontiers in Veterinary Science

    Article Title: Inactivation of highly transmissible livestock and avian viruses including influenza A and Newcastle disease virus for molecular diagnostics

    doi: 10.3389/fvets.2024.1304022

    Figure Lengend Snippet: Presence of biological material does not affect the effectiveness of PSMTM on the inactivation or nucleic acid detection of diverse viruses. Viruses were combined with species-appropriate sera or cloacal swabs as described in the Methods section. (A) Virus recovery after PrimeStore® molecular transport media (PSMTM) inactivation or PBS no-inactivation control. Virus recovery was assessed by TCID 50 titer using the cell line described in . (B) Nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation control. (C) Serial passage endpoint nucleic acid cycle threshold (CT) detection after PSMTM inactivation or PBS no-inactivation. Serial passage was completed using the cell line in and the description outlined in the Methods section. Nucleic acid was detected according to the reference in and the description in Methods. PSMTM-treated viruses were compared to the corresponding no-PSMTM control, and inactivation was assessed using the manufacturer’s recommended conditions. Significance was determined using Student’s t -test. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; ns, not significant. Error bars represent the standard error of the mean (SEM) of triplicate experiments. Low pathogenic avian influenza virus (LPAI); swine influenza virus (SIV); Newcastle disease virus (NDV); eastern equine encephalitis virus (EEEV); and bovine viral diarrhea virus (BVDV).

    Article Snippet: PrimeStore® molecular transport media (PSMTM) tubes contain proprietary reagents that inactivate both viruses and bacteria by disrupting lipid membranes and inhibiting replication machinery while stabilizing nucleic acids (PrimeStore ® , Longhorn Vaccines and Diagnostics) ( , ).

    Techniques: Virus, Control